报告题目：Saturated Stimulated Raman Scattering (SSRS) Microscopy to Break Diffraction Limit for Super-Resolution Vibrational Imaging: Principle, Technique, and Implementations
报告人：Zhiwei Huang（黄志伟），新加坡国立大学，教授
报告时间：2019年12月3日（星期二）上午10:00
报告地点：国际交叉科学研究院IRC 308会议室
邀请人：生物医学工程高精尖创新中心医用光子学研究所
报告摘要：Stimulated Raman scattering (SRS) microscopy has emerged as a powerful tool for quantitative vibrational imaging of live cells and tissue with biochemical specificity, but the spatial resolution of SRS microscopy is still diffraction limited.In this talk, I will present our recent work on saturated stimulated Raman scattering (SSRS) microscopy to break the diffraction limit for the first time for far-field super-resolution vibrational imaging in biological systems. We have established a generic semi-classical model on SSRS processes to identify the parameters of ps/fs pump and Stokes beams required for SSRS imaging. I will discuss about a virtual sinusoidal modulation method for retrieval of super-resolution SSRS image. The first experimental implementations of SSRS microscopy for enhancing the spatial resolution of SRS imaging in live cells are also presented by using a spectral focusing hyperspectral SRS system developed.With the saturation occurring in coherent Raman processes under a certain level of excitation power, we expect that SSRS technique has ability of becoming an appealing quantitative imaging tool for label-free super-resolution vibrational imaging in biological and biomedical systems.
报告人简介：
Dr Zhiwei Huang is Associate Professor with tenure in Department of Biomedical Engineering (BME), Faculty of Engineering at theNational University of Singapore (NUS). He is the Head of Optical Bioimaging Lab at BME/NUS, and a world-renowned expert in biomedical optics and microscopy imaging. His major research areas are in the fields of biomedical optics, microscopy, Raman spectroscopy and imaging, particularly centering on the development of super-resolution microscopy and nonlinear optical microscopy imaging techniques (e.g., coherent Raman scattering microscopy and multiphoton microscopy) and their applications in biomolecular imaging, as well as the development of novel fiber-optic endoscopic imaging and Raman spectroscopy, enabling early diagnosis and detection of epithelial precancer and cancer. He has pioneered in Raman endoscopy and label-free super-resolution bioimaging technologies, and published over 120 peer-reviewed articles in leading journals (e.g., Nature Photonics, Gastroenterology), and delivered over 80 plenary/keynote/invited talks worldwide. He has filed over 30 US patents with 10 licensed for commercialization. He won many international awards related to novel Raman spectroscopy and imaging techniques. His IMDX technique invented was ranked No.1 among the top 10 medical devices listed in Medica, Germany. He is the node leader in Biophotonics4Life (BP4L) Worldwide Consortium, and conference chair for Biomedical Vibrational Spectroscopy Symposium in SPIE-Photonics West-BIOS Conference in San Francisco, USA.
